Ruffolo, Hooper & Associates, MD, PA
We are a physician-owned medical practice providing comprehensive anatomic and clinical pathology services.
About Us
Ruffolo, Hooper & Associates, M.D., P.A. is a dynamic, physician-owned medical practice providing comprehensive anatomic and clinical pathology services throughout the state of Florida and extending into the U.S. Virgin Islands. Consisting of board-certified pathologists with a vast subspecialty expertise, our group serves as laboratory medical directors and staff pathologists at hospitals, ambulatory surgery centers, independent laboratories, and other patient care centers.
We give back to the local community by fulfilling academic roles as instructors and faculty at teaching institutions specializing in the training of future medical professionals. Additionally, our pathologists and pathologists’ assistants work closely with the medical residents and fellows from the Morsani College of Medicine at the University of South Florida during their rotations through affiliated teaching institutions, including Tampa General Hospital.
Our Team

Robert Ruffolo, DO
PRESIDENT
Board Certified, American Board of Pathology
Anatomic and Clinical Pathology

Kurt Stonesifer, MD
VICE PRESIDENT
Board Certified, American Board of Pathology
Anatomic and Clinical Pathology
Cytopathology
Hematopathology

Dong-Lin Xie, MD, PhD
TREASURER
Board Certified, American Board of Pathology
Anatomic and Clinical Pathology
Dermatopathology

Karen Reeves, MD
SECRETARY
Board Certified, American Board of Pathology
Anatomic and Clinical Pathology
Hematopathology

Alejandra Kalik, MD
MEMBER AT LARGE
Board Certified, American Board of Pathology
Anatomic and Clinical Pathology
Cytopathology
Keith Pautler, MD
MEMBER AT LARGE
Board Certified, American Board of Pathology
Anatomic and Clinical Pathology
Surgical Pathology
Our History
Since 1965, by upholding a continuous culture of excellence, Ruffolo, Hooper & Associates, M.D., P.A. has been and remains an industry leader in the field of pathology.
It is our mission to exceed the expectations of our referring physicians and patients by delivering timely and accurate diagnostics to support superior patient outcomes. To maintain our longstanding commitment to the community, we keep the patients and the referring clinicians at the core of our quality care and cutting-edge diagnostic services.
We pride ourselves on continued professional development, recruiting only top candidates in each subspecialty of pathology to ensure we provide our patients and clinicians with state-of-the-art healthcare.